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Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD
We engineered a class of proteins that binds selected polypeptides with high specificity and affinity. Use of the protein scaffold of Sac7d, belonging to a protein family that binds various ligands, overcomes limitations inherent in the use of antibodies as intracellular inhibitors: it lacks disulfi...
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| Autores principales: | , , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2007
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2084283/ https://ncbi.nlm.nih.gov/pubmed/17984049 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0702963104 |
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