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Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD

We engineered a class of proteins that binds selected polypeptides with high specificity and affinity. Use of the protein scaffold of Sac7d, belonging to a protein family that binds various ligands, overcomes limitations inherent in the use of antibodies as intracellular inhibitors: it lacks disulfi...

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Main Authors: Mouratou, Barbara, Schaeffer, Francis, Guilvout, Ingrid, Tello-Manigne, Diana, Pugsley, Anthony P., Alzari, Pedro M., Pecorari, Frédéric
格式: Artigo
語言:Inglês
出版: National Academy of Sciences 2007
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2084283/
https://ncbi.nlm.nih.gov/pubmed/17984049
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0702963104
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