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Metal Ions Stabilize a Dimeric Molten Globule State between the Open and Closed Forms of Malic Enzyme
Malic enzyme is a tetrameric protein with double dimer quaternary structure. In 3–5 M urea, the pigeon cytosolic NADP(+)-dependent malic enzyme unfolded and aggregated into various forms with dimers as the basic unit. Under the same denaturing conditions but in the presence of 4 mM Mn(2+), the enzym...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2084232/ https://ncbi.nlm.nih.gov/pubmed/17704184 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.111385 |
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