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Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.
The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1990
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC208398/ https://ncbi.nlm.nih.gov/pubmed/2152910 |
| Oznake: |
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