Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Seifert, H S, Ajioka, R S, Paruchuri, D, Heffron, F, So, M
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1990
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208398/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2152910/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.1.40-46.1990
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