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Analysis of Escherichia coli TonB membrane topology by use of PhoA fusions.

Alkaline phosphatase (PhoA) fusions to TonB amino acids 32, 60, 125, 207, and 239 (the carboxy terminus) all showed high PhoA activity; a PhoA fusion to TonB amino acid 12 was inactive. The full-length TonB-PhoA fusion protein was associated with the cytoplasmic membrane and retained partial TonB fu...

詳細記述

保存先:
書誌詳細
主要な著者: Roof, S K, Allard, J D, Bertrand, K P, Postle, K
フォーマット: Artigo
言語:Inglês
出版事項: 1991
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC208271/
https://ncbi.nlm.nih.gov/pubmed/1885532
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