Analysis of Escherichia coli TonB membrane topology by use of PhoA fusions.

Alkaline phosphatase (PhoA) fusions to TonB amino acids 32, 60, 125, 207, and 239 (the carboxy terminus) all showed high PhoA activity; a PhoA fusion to TonB amino acid 12 was inactive. The full-length TonB-PhoA fusion protein was associated with the cytoplasmic membrane and retained partial TonB fu...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Roof, S K, Allard, J D, Bertrand, K P, Postle, K
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1991
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208271/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1885532/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.17.5554-5557.1991
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