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Membrane topology analysis of Escherichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions.

The Escherichia coli mannitol permease catalyzes the concomitant transport and phosphorylation of D-mannitol. This 68-kDa protein consists of a membrane-bound, N-terminal domain involved in mannitol binding and translocation and a C-terminal, cytoplasmic domain responsible for mannitol phosphorylati...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Sugiyama, J E, Mahmoodian, S, Jacobson, G R
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1991
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52766/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1946374/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.21.9603
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