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Analysis of Streptococcus pneumoniae sequences cloned into Escherichia coli: effect of promoter strength and transcription terminators.

Difficulties encountered in the cloning of DNA from Streptococcus pneumoniae and other AT-rich organisms into ColE1-type Escherichia coli vectors have been proposed to be due to the presence of a large number of strong promoter-acting sequences in the donor DNA. The use of transcription terminators...

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Bibliografiske detaljer
Main Authors: Dillard, J P, Yother, J
Format: Artigo
Sprog:Inglês
Udgivet: 1991
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC208201/
https://ncbi.nlm.nih.gov/pubmed/1860821
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