Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases.
We describe the development of an expression-secretion system in Bacillus subtilis to improve the quality and quantity of the secreted foreign proteins. This system consists of a strain (WB600) deficient in six extracellular proteases and a set of sacB-based expression vectors. With the inactivation...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1991
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208183/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1907264/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.16.4952-4958.1991 |
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