Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases.

We describe the development of an expression-secretion system in Bacillus subtilis to improve the quality and quantity of the secreted foreign proteins. This system consists of a strain (WB600) deficient in six extracellular proteases and a set of sacB-based expression vectors. With the inactivation...

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Vydáno v:	J Bacteriol
Hlavní autoři:	Wu, X C, Lee, W, Tran, L, Wong, S L
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1991
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208183/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1907264/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.16.4952-4958.1991
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