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Construction of a catalytically active iron superoxide dismutase by rational protein design

The rational protein design algorithm DEZYMER was used to introduce the active site of nonheme iron superoxide dismutase (SOD) into the hydrophobic interior of the host protein, Escherichia coli thioredoxin (Trx), a protein that does not naturally contain a transition metal-binding site. Reconstitut...

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Detalhes bibliográficos
Main Authors: Pinto, Ann L., Hellinga, Homme W., Caradonna, John P.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences of the USA 1997
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC20818/
https://ncbi.nlm.nih.gov/pubmed/9159112
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