Efficient introduction of cloned mutant alleles into the Escherichia coli chromosome.
An efficient method for moving mutations in cloned Escherichia coli DNA from plasmid vectors to the bacterial chromosome was developed. Cells carrying plasmids that had been mutated by the insertion of a resistance gene were infected with lambda phage containing homologous cloned DNA, and resulting...
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| Published in: | J Bacteriol |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1991
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207830/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1826503/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.8.2633-2638.1991 |
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