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Optimized bacterial expression of myocilin proteins and functional comparison of bacterial and eukaryotic myocilins

PURPOSE: To maximize the expression level of myocilin and its truncated proteins in Escherichia coli (E. coli) and to examine the biological effects of bacterially expressed myocilin as compared to eukaryotic myocilin on cultured human trabecular meshwork (TM) cells. METHODS: Myocilin full length (1...

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Detalhes bibliográficos
Main Authors: Park, Bum-Chan, Shen, Xiang, Fautsch, Michael P., Tibudan, Martin, Johnson, Douglas H., Yue, Beatrice Y. J. T.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2077819/
https://ncbi.nlm.nih.gov/pubmed/16902400
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