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Cloning and sequencing of the 7 alpha-hydroxysteroid dehydrogenase gene from Escherichia coli HB101 and characterization of the expressed enzyme.

The 7 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) gene from Escherichia coli HB101 was cloned and expressed in E. coli DH1. The hybrid plasmid pSD1, with a 2.8-kbp insert of chromosomal DNA at the BamHI site of pBR322, was subcloned into pUC19 to construct plasmid pSD3. The entire nucleotide s...

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Publicado no:J Bacteriol
Principais autores: Yoshimoto, T, Higashi, H, Kanatani, A, Lin, X S, Nagai, H, Oyama, H, Kurazono, K, Tsuru, D
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1991
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207764/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2007545/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.7.2173-2179.1991
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