Cloning and sequencing of the 7 alpha-hydroxysteroid dehydrogenase gene from Escherichia coli HB101 and characterization of the expressed enzyme.

The 7 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) gene from Escherichia coli HB101 was cloned and expressed in E. coli DH1. The hybrid plasmid pSD1, with a 2.8-kbp insert of chromosomal DNA at the BamHI site of pBR322, was subcloned into pUC19 to construct plasmid pSD3. The entire nucleotide s...

Description complète

Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Yoshimoto, T, Higashi, H, Kanatani, A, Lin, X S, Nagai, H, Oyama, H, Kurazono, K, Tsuru, D
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1991
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207764/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2007545/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.7.2173-2179.1991
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!