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Stochastic inhibitor release and binding from single-enzyme molecules

Inhibition kinetics of single-β-galactosidase molecules with the slow-binding inhibitor d-galactal have been characterized by segregating individual enzyme molecules in an array of 50,000 ultra small reaction containers and observing substrate turnover changes with fluorescence microscopy. Inhibited...

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Autors principals: Gorris, Hans H., Rissin, David M., Walt, David R.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2007
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2077050/
https://ncbi.nlm.nih.gov/pubmed/17965235
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0705411104
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