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Stochastic inhibitor release and binding from single-enzyme molecules
Inhibition kinetics of single-β-galactosidase molecules with the slow-binding inhibitor d-galactal have been characterized by segregating individual enzyme molecules in an array of 50,000 ultra small reaction containers and observing substrate turnover changes with fluorescence microscopy. Inhibited...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2077050/ https://ncbi.nlm.nih.gov/pubmed/17965235 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0705411104 |
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