A Freeze Substitution Fixation-Based Gold Enlarging Technique for EM Studies of Endocytosed Nanogold-Labeled Molecules

We have developed methods to locate individual ligands that can be used for electron microscopy studies of dynamic events during endocytosis and subsequent intracellular trafficking. The methods are based on enlargement of 1.4 nm Nanogold attached to an endocytosed ligand. Nanogold, a small label th...

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Main Authors: He, Wanzhong, Kivork, Christine, Machinani, Suman, Morphew, Mary K., Gail, Anna M., Tesar, Devin B., Tiangco, Noreen E., McIntosh, J. Richard, Bjorkman, Pamela J.
Format: Artigo
Jezik:Inglês
Izdano: 2007
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2076746/
https://ncbi.nlm.nih.gov/pubmed/17723309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2007.07.004
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