A mer-lux transcriptional fusion for real-time examination of in vivo gene expression kinetics and promoter response to altered superhelicity.

We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yield...

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Pubblicato in:	J Bacteriol
Autori principali:	Condee, C W, Summers, A O
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1992
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207548/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1334070/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.24.8094-8101.1992
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