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A mer-lux transcriptional fusion for real-time examination of in vivo gene expression kinetics and promoter response to altered superhelicity.

We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yield...

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Dades bibliogràfiques
Publicat a:J Bacteriol
Autors principals: Condee, C W, Summers, A O
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1992
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207548/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1334070/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.24.8094-8101.1992
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