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A novel approach to insertional mutagenesis of Haemophilus influenzae.

Insertional mutagenesis of the Haemophilus influenzae chromosome was accomplished by a novel method employing a 2.2-kbp element, TSTE. This element, consisting of the neo gene of Tn5 flanked by Haemophilus-specific uptake sequences, was ligated to circularized chromosomal fragments before transforma...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Sharetzsky, C, Edlind, T D, LiPuma, J J, Stull, T L
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1991
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207296/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1847369/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.4.1561-1564.1991
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