A novel approach to insertional mutagenesis of Haemophilus influenzae.

Insertional mutagenesis of the Haemophilus influenzae chromosome was accomplished by a novel method employing a 2.2-kbp element, TSTE. This element, consisting of the neo gene of Tn5 flanked by Haemophilus-specific uptake sequences, was ligated to circularized chromosomal fragments before transforma...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Sharetzsky, C, Edlind, T D, LiPuma, J J, Stull, T L
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207296/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1847369/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.4.1561-1564.1991
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