Is strong hydrogen bonding in the transition state enough to account for the observed rate acceleration in a mutant of papain?
Nitriles are good inhibitors for the cysteine protease papain. However, a single amino acid mutation (Gln-19 → Glu-19) in the active site makes the mutant enzyme a good catalyst for nitrile hydrolysis. A theoretical approach was used to examine the differential transition state stabilization in the...
Na minha lista:
| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1997
|
| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC20714/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9113981/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.9.4285 |
| Tags: |
Sem tags, seja o primeiro a adicionar uma tag!
|