Cloning, sequencing, and enhanced expression of the dihydropteroate synthase gene of Escherichia coli MC4100.

The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased expression of the enzyme was realized by using a T7 expression system. The enzyme was purified and cryst...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Dallas, W S, Gowen, J E, Ray, P H, Cox, M J, Dev, I K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207134/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1522070/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.18.5961-5970.1992
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