Cloning, sequencing, and enhanced expression of the dihydropteroate synthase gene of Escherichia coli MC4100.

The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased expression of the enzyme was realized by using a T7 expression system. The enzyme was purified and cryst...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Dallas, W S, Gowen, J E, Ray, P H, Cox, M J, Dev, I K
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1992
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207134/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1522070/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.18.5961-5970.1992
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