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Modification of the properties of a Ruminococcus albus endo-1,4-beta-glucanase by gene truncation.

An endo-1,4-beta-glucanase (EgI) gene isolated from Ruminococcus albus was deleted at the 5'-flanking region by gene truncation or at the 3'-flanking region by insertion of an omega (omega) fragment with a universal stop codon at the EcoRI or BamHI site. These modified genes were integrated into pUC...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Ohmiya, K, Deguchi, H, Shimizu, S
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207054/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1987156/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.2.636-641.1991
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