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Modification of the properties of a Ruminococcus albus endo-1,4-beta-glucanase by gene truncation.
An endo-1,4-beta-glucanase (EgI) gene isolated from Ruminococcus albus was deleted at the 5'-flanking region by gene truncation or at the 3'-flanking region by insertion of an omega (omega) fragment with a universal stop codon at the EcoRI or BamHI site. These modified genes were integrate...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
1991
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC207054/ https://ncbi.nlm.nih.gov/pubmed/1987156 |
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