Cloning of the cellulase gene from Ruminococcus albus and its expression in Escherichia coli.
The gene for cellulase from Ruminococcus albus F-40 was cloned in Escherichia coli HB101 with pBR322. A 3.4-kilobase-pair HindIII fragment encoding cellulase hybridized with the chromosomal DNA of R. albus. The Ouchterlony double-fusion test gave a single precipitation line between the cloned enzyme...
Guardat en:
| Publicat a: | Appl Environ Microbiol |
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| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1988
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202688/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2843095/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.6.1511-1515.1988 |
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