Cloning of the cellulase gene from Ruminococcus albus and its expression in Escherichia coli.

The gene for cellulase from Ruminococcus albus F-40 was cloned in Escherichia coli HB101 with pBR322. A 3.4-kilobase-pair HindIII fragment encoding cellulase hybridized with the chromosomal DNA of R. albus. The Ouchterlony double-fusion test gave a single precipitation line between the cloned enzyme...

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Main Authors: Ohmiya, K, Nagashima, K, Kajino, T, Goto, E, Tsukada, A, Shimizu, S
Formato: Artigo
Idioma:Inglês
Publicado: 1988
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC202688/
https://ncbi.nlm.nih.gov/pubmed/2843095
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