Cloning of the cellulase gene from Ruminococcus albus and its expression in Escherichia coli.

The gene for cellulase from Ruminococcus albus F-40 was cloned in Escherichia coli HB101 with pBR322. A 3.4-kilobase-pair HindIII fragment encoding cellulase hybridized with the chromosomal DNA of R. albus. The Ouchterlony double-fusion test gave a single precipitation line between the cloned enzyme...

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izdano v:	Appl Environ Microbiol
Principais autores:	Ohmiya, K, Nagashima, K, Kajino, T, Goto, E, Tsukada, A, Shimizu, S
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1988
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202688/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2843095/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.6.1511-1515.1988
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