Gene replacement in Lactobacillus helveticus.

An efficient method for gene replacement in Lactobacillus helveticus CNRZ32 was developed by utilizing pSA3 as an integration vector. This plasmid is stably maintained in CNRZ32 at 37 degrees C but is unstable at 45 degrees C. This method consisted of a two-step gene-targeting technique: (i) chromos...

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Dades bibliogràfiques

Publicat a:	J Bacteriol
Autors principals:	Bhowmik, T, Fernández, L, Steele, J L
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1993
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206732/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8104928/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.19.6341-6344.1993
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