Gene replacement in Lactobacillus helveticus.

An efficient method for gene replacement in Lactobacillus helveticus CNRZ32 was developed by utilizing pSA3 as an integration vector. This plasmid is stably maintained in CNRZ32 at 37 degrees C but is unstable at 45 degrees C. This method consisted of a two-step gene-targeting technique: (i) chromos...

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Argitaratua izan da:	J Bacteriol
Egile Nagusiak:	Bhowmik, T, Fernández, L, Steele, J L
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	American Society for Microbiology (ASM) 1993
Gaiak:	Research Article
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206732/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8104928/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.19.6341-6344.1993
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