Transposon mutagenesis in Actinobacillus pleuropneumoniae with a Tn10 derivative.

A transposon mutagenesis procedure functional in the gram-negative swine pathogen Actinobacillus pleuropneumoniae was developed for the first time. The technique involved the use of a suicide conjugative plasmid, pLOF/Km, carrying a mini-Tn10 with an isopropyl-beta-D-thiogalactopyranoside (IPTG)-ind...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Tascon, R I, Rodriguez-Ferri, E F, Gutierrez-Martin, C B, Rodriguez-Barbosa, I, Berche, P, Vazquez-Boland, J A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206634/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8396122/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.17.5717-5722.1993
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