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Transposon mutagenesis in Actinobacillus pleuropneumoniae with a Tn10 derivative.

A transposon mutagenesis procedure functional in the gram-negative swine pathogen Actinobacillus pleuropneumoniae was developed for the first time. The technique involved the use of a suicide conjugative plasmid, pLOF/Km, carrying a mini-Tn10 with an isopropyl-beta-D-thiogalactopyranoside (IPTG)-ind...

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Sonraí Bibleagrafaíochta
Main Authors: Tascon, R I, Rodriguez-Ferri, E F, Gutierrez-Martin, C B, Rodriguez-Barbosa, I, Berche, P, Vazquez-Boland, J A
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1993
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC206634/
https://ncbi.nlm.nih.gov/pubmed/8396122
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