New method for gene disruption in Salmonella typhimurium: construction and characterization of an ada-deletion derivative of Salmonella typhimurium TA1535.
A new method for gene disruption in Salmonella typhimurium was developed. The key steps of this method are to produce restriction fragments with compatible ends, preligate to produce concatemers, and then transform by electrotransformation. We developed and used this method to construct a mutant of...
Enregistré dans:
| Publié dans: | J Bacteriol |
|---|---|
| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1993
|
| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206610/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8366039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.17.5539-5547.1993 |
| Tags: |
Pas de tags, Soyez le premier à ajouter un tag!
|