New method for gene disruption in Salmonella typhimurium: construction and characterization of an ada-deletion derivative of Salmonella typhimurium TA1535.
A new method for gene disruption in Salmonella typhimurium was developed. The key steps of this method are to produce restriction fragments with compatible ends, preligate to produce concatemers, and then transform by electrotransformation. We developed and used this method to construct a mutant of...
Guardat en:
| Publicat a: | J Bacteriol |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1993
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206610/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8366039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.17.5539-5547.1993 |
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