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Quantitation of Dam methyltransferase in Escherichia coli.

An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase. IMAGES:

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Boye, E, Marinus, M G, Løbner-Olesen, A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206566/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1537808/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.5.1682-1685.1992
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