Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon.
The mini-Tn10 transposon (delta 16 delta 17Tn10) confers tetracycline resistance. When inserted between a gene and its promoter, it blocks transcription and prevents expression of that gene. Tetracycline in the medium induces divergent transcription of the tetA and tetR genes within the transposon,...
Sparad:
| I publikationen: | J Bacteriol |
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| Huvudupphov: | , , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
American Society for Microbiology (ASM)
1992
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206550/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1537799/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.5.1544-1553.1992 |
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