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Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon.

The mini-Tn10 transposon (delta 16 delta 17Tn10) confers tetracycline resistance. When inserted between a gene and its promoter, it blocks transcription and prevents expression of that gene. Tetracycline in the medium induces divergent transcription of the tetA and tetR genes within the transposon,...

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Detalles Bibliográficos
Main Authors: Takiff, H E, Baker, T, Copeland, T, Chen, S M, Court, D L
Formato: Artigo
Idioma:Inglês
Publicado: 1992
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC206550/
https://ncbi.nlm.nih.gov/pubmed/1537799
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