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Real-time closed tube single nucleotide polymorphism (SNP) quantification in pooled samples by quencher extension (QEXT)
Quencher extension (QEXT) is a novel single step closed tube real-time method to quantify SNPs using reporters and quenchers in combination with primer extension. A probe with a 5′-reporter dye is single base extended with a dideoxy nucleotide containing a quencher dye if the target SNP allele is pr...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2003
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC206483/ https://ncbi.nlm.nih.gov/pubmed/14500845 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng118 |
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