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Real-time closed tube single nucleotide polymorphism (SNP) quantification in pooled samples by quencher extension (QEXT)

Quencher extension (QEXT) is a novel single step closed tube real-time method to quantify SNPs using reporters and quenchers in combination with primer extension. A probe with a 5′-reporter dye is single base extended with a dideoxy nucleotide containing a quencher dye if the target SNP allele is pr...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Principais autores: Rudi, Knut, Holck, Askild L.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2003
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206483/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14500845/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng118
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