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Cloning and sequencing of the gene coding for alcohol dehydrogenase of Bacillus stearothermophilus and rational shift of the optimum pH.

Using Bacillus subtilis as a host and pTB524 as a vector plasmid, we cloned the thermostable alcohol dehydrogenase (ADH-T) gene (adhT) from Bacillus stearothermophilus NCA1503 and determined its nucleotide sequence. The deduced amino acid sequence (337 amino acids) was compared with the sequences of...

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Bibliografske podrobnosti
izdano v:J Bacteriol
Principais autores: Sakoda, H, Imanaka, T
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 1992
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206437/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1735726/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.4.1397-1402.1992
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