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Purification and characterization of EpiD, a flavoprotein involved in the biosynthesis of the lantibiotic epidermin.

The plasmid-encoded epidermin biosynthesis gene, epiD, of Staphylococcus epidermidis Tü3298 was expressed in Escherichia coli by using both the malE fusion system and the T7 RNA polymerase-promoter system. EpiD was identified by Western blotting (immunoblotting) with anti-maltose-binding protein (MB...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Kupke, T, Stevanović, S, Sahl, H G, Götz, F
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206373/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1644762/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.16.5354-5361.1992
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