Purification and characterization of EpiD, a flavoprotein involved in the biosynthesis of the lantibiotic epidermin.
The plasmid-encoded epidermin biosynthesis gene, epiD, of Staphylococcus epidermidis Tü3298 was expressed in Escherichia coli by using both the malE fusion system and the T7 RNA polymerase-promoter system. EpiD was identified by Western blotting (immunoblotting) with anti-maltose-binding protein (MB...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1992
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206373/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1644762/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.16.5354-5361.1992 |
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