A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction.
An internal fragment of the recA gene from eight gram-positive organisms has been amplified by using degenerate primers in a polymerase chain reaction. The internal 348- or 360-bp recA DNA segments from Bacillus subtilis, Clostridium acetobutylicum, Lactobacillus bulgaricus, Lactobacillus helveticus...
Uloženo v:
| Vydáno v: | J Bacteriol |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1992
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206342/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1629178/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.15.5171-5175.1992 |
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