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An efficient screen for peroxisome-deficient mutants of Pichia pastoris.

We describe a rapid and efficient screen for peroxisome-deficient (per) mutants in the yeast Pichia pastoris. The screen relies on the unusual ability of P. pastoris to grow on two carbon sources, methanol and oleic acid, both of which absolutely require peroxisomes to be metabolized. A collection o...

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Dades bibliogràfiques
Publicat a:J Bacteriol
Autors principals: Liu, H, Tan, X, Veenhuis, M, McCollum, D, Cregg, J M
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1992
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206307/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1629154/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.15.4943-4951.1992
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