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An efficient screen for peroxisome-deficient mutants of Pichia pastoris.

We describe a rapid and efficient screen for peroxisome-deficient (per) mutants in the yeast Pichia pastoris. The screen relies on the unusual ability of P. pastoris to grow on two carbon sources, methanol and oleic acid, both of which absolutely require peroxisomes to be metabolized. A collection o...

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Udgivet i:J Bacteriol
Principais autores: Liu, H, Tan, X, Veenhuis, M, McCollum, D, Cregg, J M
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206307/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1629154/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.15.4943-4951.1992
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