Dissection of the Salmonella typhimurium genome by use of a Tn5 derivative carrying rare restriction sites.

A polylinker with rare restriction sites was introduced into a mini-Tn5 derivative. These sites include M.XbaI-DpnI (TCTAGATCTAGA), which is rare in most bacterial genomes, SwaI (ATTTAAAT) and PacI (TTAATTAA), which are rare in G+C-rich genomes, NotI (GCGGCCGC) and SfiI (GGCCN5GGCC), which are rare...

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Bibliografische gegevens

Gepubliceerd in:	J Bacteriol
Hoofdauteurs:	Wong, K K, McClelland, M
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	American Society for Microbiology (ASM) 1992
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206072/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1317382/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.11.3807-3811.1992
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