Dissection of the Salmonella typhimurium genome by use of a Tn5 derivative carrying rare restriction sites.
A polylinker with rare restriction sites was introduced into a mini-Tn5 derivative. These sites include M.XbaI-DpnI (TCTAGATCTAGA), which is rare in most bacterial genomes, SwaI (ATTTAAAT) and PacI (TTAATTAA), which are rare in G+C-rich genomes, NotI (GCGGCCGC) and SfiI (GGCCN5GGCC), which are rare...
Guardado en:
| Publicado en: | J Bacteriol |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1992
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206072/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1317382/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.11.3807-3811.1992 |
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