Dissection of the Salmonella typhimurium genome by use of a Tn5 derivative carrying rare restriction sites.

A polylinker with rare restriction sites was introduced into a mini-Tn5 derivative. These sites include M.XbaI-DpnI (TCTAGATCTAGA), which is rare in most bacterial genomes, SwaI (ATTTAAAT) and PacI (TTAATTAA), which are rare in G+C-rich genomes, NotI (GCGGCCGC) and SfiI (GGCCN5GGCC), which are rare...

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Bibliographic Details
Main Authors: Wong, K K, McClelland, M
Format: Artigo
Language:Inglês
Published: 1992
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC206072/
https://ncbi.nlm.nih.gov/pubmed/1317382
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