Heterologous expression and secretion of a Streptomyces scabies esterase in Streptomyces lividans and Escherichia coli.

The esterase gene from Streptomyces scabies FL1 was cloned and expressed in Streptomyces lividans on plasmids pIJ486 and pIJ702. In S. lividans, the esterase gene was expressed during later stages of growth and was regulated by zinc, as is seen with S. scabies. The 36-kDa secreted form of the estera...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Hale, V, McGrew, M, Carlson, B, Schottel, J L
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1992
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205878/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1556063/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.8.2431-2439.1992
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