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Cloning and expression in streptomyces lividans of antibiotic resistance genes derived from Escherichia coli.

Hybrid plasmids that replicate in both Escherichia coli and Streptomyces lividans were constructed in vitro by joining the E. coli-derived plasmid pACYC184 or pACYC177, at their BamHI or PstI restriction site, respectively, to S. lividans plasmid pSLP111. After introduction of the composite replicon...

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Bibliographic Details
Main Authors: Schottel, J L, Bibb, M J, Cohen, S N
Format: Artigo
Language:Inglês
Published: 1981
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC217091/
https://ncbi.nlm.nih.gov/pubmed/7012122
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