Cloning, mutagenesis, and physiological effect of a hydroxypyruvate reductase gene from Methylobacterium extorquens AM1.

The gene encoding the serine cycle hydroxypyruvate reductase of Methylobacterium extorquens AM1 was isolated by using a synthetic oligonucleotide with a sequence based on a known N-terminal amino acid sequence. The cloned gene was inactivated by insertion of a kanamycin resistance gene, and recombin...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Chistoserdova, L V, Lidstrom, M E
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1992
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205678/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1729225/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.1.71-77.1992
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