Cloning, mutagenesis, and physiological effect of a hydroxypyruvate reductase gene from Methylobacterium extorquens AM1.
The gene encoding the serine cycle hydroxypyruvate reductase of Methylobacterium extorquens AM1 was isolated by using a synthetic oligonucleotide with a sequence based on a known N-terminal amino acid sequence. The cloned gene was inactivated by insertion of a kanamycin resistance gene, and recombin...
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| Published in: | J Bacteriol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1992
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205678/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1729225/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.1.71-77.1992 |
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